Essential role of the TRIC-B channel in Ca2+ handling of alveolar epithelial cells and in perinatal lung maturation
Author:
Yamazaki Daiju1, Komazaki Shinji2, Nakanishi Hiroki3, Mishima Aya1, Nishi Miyuki1, Yazawa Masayuki1, Yamazaki Tetsuo1, Taguchi Ryo3, Takeshima Hiroshi1
Affiliation:
1. Department of Biological Chemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606-8501, Japan. 2. Department of Anatomy, Saitama Medical University, Saitama 350-0495,Japan. 3. Department of Metabolome, Faculty of Medicine, University of Tokyo, Tokyo 113-0033, Japan.
Abstract
TRIC channels function as monovalent cation-specific channels that mediate counter ion movements coupled with ryanodine receptor-mediated Ca2+release from intracellular stores in muscle cells. Mammalian tissues differentially contain two TRIC channel subtypes: TRIC-A is abundantly expressed in excitable cells, whereas TRIC-B is ubiquitously expressed throughout tissues. Here, we report the physiological role of TRIC-B channels in mouse perinatal development. TRIC-B-knockout neonates were cyanotic owing to respiratory failure and died shortly after birth. In the mutant neonates,the deflated lungs exhibited severe histological defects, and alveolar type II epithelial cells displayed ultrastructural abnormalities. The metabolic conversion of glycogen into phospholipids was severely interrupted in the mutant type II cells, and surfactant phospholipids secreted into the alveolar space were insufficient in the mutant neonates. Moreover, the mutant type II cells were compromised for Ca2+ release mediated by inositol-trisphosphate receptors, despite Ca2+ overloading in intracellular stores. Our results indicate that TRIC-B channels take an active part in Ca2+ signalling to establish specialised functions in type II cells and are thus essential for perinatal lung maturation.
Publisher
The Company of Biologists
Subject
Developmental Biology,Molecular Biology
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