Human achilles tendon plasticity in response to cyclic strain: effect of rate and duration

Abstract

Abstract High strain magnitude and low strain frequency are important stimuli for tendon adaptation. Increasing the rate and duration of the applied strain may enhance the adaptive responses. Therefore, our purpose was to investigate the effect of strain rate and duration on Achilles tendon adaptation. The study included two experimental groups (n=14 and n=12) and a control group (n=13). The participants of the experimental groups exercised (14 weeks, 4x/week) according to a reference protocol, featuring a high strain magnitude (~6.5%) and a low strain frequency (0.17 Hz, 3 s loading/3 s relaxation) on one leg and with either a higher strain rate (one-legged jumps) or a longer strain duration (12 s loading) on the other leg. The strain magnitude and loading volume were similar in all protocols. Before and after the interventions the tendon stiffness, Young’s modulus and cross-sectional area were examined using magnetic resonance imaging, ultrasound and dynamometry. The reference and long strain duration protocols induced significantly increased (p<0.05) tendon stiffness (57% vs. 25%), cross-sectional area (4.2% vs. 5.3%) and Young’s modulus (51% vs. 17%). The increases in tendon stiffness and Young’s modulus were higher in the reference protocol. Although region-specific tendon hypertrophy was also detected after the high strain rate training, there was only a tendency of increased stiffness (p=0.08) and Young's modulus (p=0.09). The control group did not show any changes (p>0.05). The results provide evidence that a high strain magnitude, an appropriate strain duration and repetitive loading are essential components for an efficient adaptive stimulus for tendons.