Gestation increases the energetic cost of breathing in the lizard, Tiliqua rugosa

Abstract

Summary High gestational loads result in fetuses that occupy a large proportion of the body cavity and may compress maternal organs. Compression of the lungs results in alterations in breathing patterns during gestation which may affect the oxidative cost of breathing. In this study, the oxidative cost of breathing during gestation was determined in the viviparous skink, Tiliqua rugosa. Radiographic imaging showed progressive lung compression during gestation and a 30% reduction in the lung compression index (rib number at which the caudal margin of the lung was imaged). Pneumotachography and open flow respirometry were used to measure breathing patterns and metabolic rates. Gestation induced a two fold increase in minute ventilation via increases in breathing frequency but no change in inspired tidal volume. The rates of O2 consumption and CO2 production did not change significantly during gestation. Together, these results suggest that a relative hyperventilation occurs during gestation in Tiliqua rugosa. This relative hyperventilation suggests that diffusion and/or perfusion limitations may exist at the lung during gestation. The oxidative cost of breathing was estimated as a percentage of resting metabolic rate using hypercapnia to stimulate ventilation at different stages of pregnancy. The oxidative cost of breathing in non pregnant lizards was 19.96±3.85% and increased 3 fold to 62.80±10.11% during late gestation. This significant increase in the oxidative cost of breathing may have significant consequences for energy budgets during gestation.