The C. elegans gene gvd-1 promotes late larval development and germ cell proliferation

Author:

Ezhil Buvani Anbalagan Pon12,Subramaniam Kuppuswamy2ORCID

Affiliation:

1. Indian Institute of Technology–Madras 1 Department of Biotechnology , , Chennai 600036 , India

2. Indian Institute of Technology 2 Department of Biological Sciences & Bioengineering , , Kanpur 208016 , India

Abstract

ABSTRACT Limiting maternal resources necessitates deferring the development of adult-specific structures, notably the reproductive structures, to the postembryonic phase. These structures form postembryonically from blast cells generated during embryogenesis. A close coordination of developmental timing and pattern among the various postembryonic cell lineages is essential to form a functional adult. Here, we show that the C. elegans gene gvd-1 is essential for the development of several structures that form during the late larval stages. In gvd-1 mutant animals, blast cells that normally divide during the late larval stages (L3 and L4) fail to divide. In addition, germ cell proliferation is also severely reduced in these animals. Expression patterns of relevant reporter transgenes revealed a delay in G1/S transition in the vulval precursor cell P6.p and cytokinesis failure in seam cells in gvd-1 larvae. Our analyses of GVD-1::GFP transgenes indicate that GVD-1 is expressed in both soma and germ line, and functions in both. Sequence comparisons revealed that the sequence of gvd-1 is conserved only among nematodes, which does not support a broadly conserved housekeeping function for gvd-1. Instead, our results indicate a crucial role for gvd-1 that is specific to the larval development of nematodes.

Funder

National Institutes of Health

Ministry of Science and Technology

Publisher

The Company of Biologists

Subject

General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology

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