Studies on the de novo formation of centrioles: aster formation in the activated eggs of sea urchin

Abstract

Aster formation was studied in sea-urchin eggs artificially activated by Loeb's double method. The number of asters found in an activated egg ranged from 15–40. Observation by electron microscopy revealed the presence of a centriole in a high percentage of isolated asters, strongly suggesting that these centrioles are formed de novo in the activated eggs. Using negatively stained specimens, which were isolated with 5% hexylene glycol solution containing buffer and EGTA, the whole aster could be examined by electron microscopy. A microtubular framework in the aster, extending radially from the astral centre was observed. The effect of mitotic inhibitors on aster formation was found to be similar to that on cell division.