Affiliation:
1. UMR 7622, CNRS-Université Paris VI, 9, Quai St. Bernard, 75252 Paris Cedex 05, France
Abstract
Apoptosis plays a major role in vertebrate and invertebrate development. The adult Drosophila thoracic microchaete is a mechanosensory organ whose development has been extensively studied as a model of how cell division and cell determination intermingle. This sensory organ arises from a cell lineage that produces a glial cell and four other cells that form the organ. In this study, using an in vivo approach as well as fixed material, we show that the glial cell undergoes nucleus fragmentation shortly after birth. Fragmentation was blocked after overexpression of the caspase inhibitor p35 or removal of the pro-apoptotic genes reaper, hid and grim,showing that the glial cell undergoes apoptosis. Moreover, it seems that fragments are eliminated from the epithelium by mobile macrophages. Forcing survival of the glial cells induces precocious axonal outgrowth but does not affect final axonal patterning and connectivity. However, under these conditions, glial cells do not fragment but leave the epithelium by a mechanism that is reminiscent of cell competition. Finally, we present evidences showing that glial cells are committed to apoptosis independently ofgcm and prospero expression. We suggest that apoptosis is triggered by a cell autonomous mechanism.
Publisher
The Company of Biologists
Subject
Developmental Biology,Molecular Biology
Reference48 articles.
1. Adachi-Yamada, T., Fujimura-Kamada, K., Nishida, Y. and Matsumoto, K. (1999). Distortion of proximodistal information causes JNK-dependent apoptosis in Drosophila wing.Nature400,166-169.
2. Bellaiche, Y., Gho, M., Kaltschmidt, J. A., Brand, A. H. and Schweisguth, F. (2001). Frizzled regulates localization of cell-fate determinants and mitotic spindle rotation during asymmetric cell division. Nat. Cell Biol.3, 50-57.
3. Bellaiche, Y. and Schweisguth, F. (2001). Lineage diversity in the Drosophila nervous system. Curr. Opin. Genet. Dev.11,418-423.
4. Brand, A. H. and Perrimon, N. (1993). Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development118,401-415.
5. Brewster, R. and Bodmer, R. (1995). Origin and specification of type II sensory neurons in Drosophila.Development121,2923-2936.
Cited by
72 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献