Affiliation:
1. Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur, Bangalore, India
2. University of Colorado, Anschutz Medical Campus, Department of Cell and Developmental Biology, USA
Abstract
Autophagy is a conserved cellular degradation pathway wherein double-membrane vesicle called autophagosomes capture long-lived proteins and damaged or superfluous organelles and deliver them to the lysosome for degradation. Septins are conserved GTP-binding proteins involved in many cellular processes, including phagocytosis and autophagy of intracellular bacteria, but no role in general autophagy was known. In budding yeast, septins polymerize into ring-shaped arrays of filaments required for cytokinesis. In an unbiased genetic screen and in subsequent targeted analysis, we found autophagy defects in septin mutants, and co-localized septins in rings at the pre-autophagosomal structure (PAS) and on autophagosomes where they physically interact with the autophagy proteins Atg8 and Atg9. Pre-assembled septin complexes relocalized to the PAS upon autophagy induction. Septin-mutant cells contained fewer autophagocytic structures, even when autophagosome degradation was blocked, and a mutation (atg1Δ) blocking PAS maturation, but not initial PAS assembly, decreased septin localization to the PAS. Our findings support a role for septins in the early stages of budding yeast autophagy, during autophagosome formation.
Publisher
The Company of Biologists
Cited by
21 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献