Phenotypic characterization ofDrosophila idamutants: defining the role of APC5 in cell cycle progression
Author:
Bentley A. M.1, Williams Byron C.2, Goldberg Michael L.2, Andres Andrew J.1
Affiliation:
1. Department of Molecular Pharmacology and Biological Chemistry, Robert H. Lurie Comprehensive Cancer Center, Northwestern University, 303 East Chicago Avenue,Chicago, IL 60611-3093, USA 2. Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14853-2703, USA
Abstract
We have cloned and characterized the ida gene that is required for proliferation of imaginal disc cells during Drosophila development. IDA is homologous to APC5, a subunit of the anaphase-promoting complex(APC/cyclosome). ida mRNA is detected in most cell types throughout development, but it accumulates to its highest levels during early embryogenesis. A maternal component of IDA is required for the production of eggs and viable embryos. Homozygous ida mutants display mitotic defects: they die during prepupal development, lack all mature imaginal disc structures, and have abnormally small optic lobes. Cytological observations show that ida mutant brains have a high mitotic index and many imaginal cells contain an aneuploid number of aberrant overcondensed chromosomes. However, cells are not stalled in metaphase, as mitotic stages in which chromosomes are orientated at the equatorial plate are never observed. Interestingly, some APC/C-target substrates such as cyclin B are not degraded in ida mutants, whereas others controlling sister-chromatid separation appear to be turned over. Taken together, these results suggest a model in which IDA/APC5 controls regulatory subfunctions of the anaphase-promoting complex.
Publisher
The Company of Biologists
Reference72 articles.
1. Adams, M. D., Celniker, S. E., Holt, R. A., Evans, C. A.,Gocayne, J. D., Amanatides, P. G., Scherer, S. E., Li, P. W., Hoskins, R. A.,Galle, R. F. et al. (2000). The genome sequence of Drosophila melanogaster.Science287, 2185-2195. 2. Altschul, S. F., Madden, T. L.,Schäffer, A. A., Zhang, J., Zhang, Z., Miller,W. and Lipman, D. J. (1997). Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic. Acid Res.25, 3389-3402. 3. Amon, A., Irniger, S. and Nasmyth, K. (1994). Closing the cell cycle circle in yeast: G2 cyclin proteolysis initiated at mitosis persists until the activation of G1 cyclins in the next cycle.Cell77, 1037-1050. 4. Andres, A. J. and Thummel, C. S. (1995). The Drosophila 63F early puff contains E63-1, an ecdysone-inducible gene that encodes a novel Ca2+-binding protein.Development121, 2667-2679. 5. Basu, J., Bousbaa, H., Logarinho, E., Li, Z., Williams, B. C.,Lopes, C., Sunkel, C. E. and Goldberg, M. L. (1999). Mutations in the essential spindle checkpoint gene bub1 cause chromosome missegregation and fail to block apoptosis in Drosophila.J. Cell Biol.146, 13-28.
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