Affiliation:
1. Department of Zoology, University of Cambridge; Institute of Oceanography, Dalhousie University, Halifax, N.S., Canada
Abstract
1. The Roughton & Root (1945) modification of the Van Slyke method for measuring haemoglobin content of blood as carboxyhaemoglobin is satisfactory for fish blood, provided at least 0.1 ml. is available.
2. Measurements of iron content are of doubtful value in estimating the oxygen capacity of fish blood.
3. The following photometric methods of determining haemoglobin content were found to be unreliable with fish blood: acid haematin, alkaline haematin and cyanhaematin.
4. The following photometric procedures gave satisfactory measurements of the haemoglobin content of fish blood: Haldane (carboxyhaemoglobin), oxyhaemoglobin and pyridine haemochromogen.
5. Blood from goldfish acclimatized to temperatures near the extremes of their thermal range shows no difference from normal blood in oxygen capacity.
Publisher
The Company of Biologists
Subject
Insect Science,Molecular Biology,Animal Science and Zoology,Aquatic Science,Physiology,Ecology, Evolution, Behavior and Systematics
Cited by
32 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献