TGF-beta/BMP superfamily members, Gbb-60A and Dpp, cooperate to provide pattern information and establish cell identity in the Drosophila wing

Abstract

Within a developing organism, cells receive many signals which control their proliferation, fate specification and differentiation. One group of such proteins is the TGF-beta/BMP class of related signaling molecules. Based on expression studies, multiple members of this class of ligands must impinge upon the same cells of a developing tissue; however, the role that multiple TGF-beta/BMP ligands may play in directing the development of such a tissue is not understood. Here we provide evidence that multiple BMPs are required for growth and patterning of the Drosophila wing. The Drosophila BMP gene, gbb-60A, exhibits a requirement in wing morphogenesis distinct from that shown previously for dpp, a well-characterized Drosophila BMP member. gbb-60A mutants exhibit a loss of pattern elements from the wing, particularly those derived from cells in the posterior compartment, consistent with the gbb-60A RNA and protein expression pattern. Based on genetic analysis and expression studies, we conclude that Gbb-60A must signal primarily as a homodimer to provide patterning information in the wing imaginal disc. We demonstrate that gbb-60A and dpp genetically interact and that specific aspects of this interaction are synergistic while others are antagonistic. We propose that the positional information received by a cell at a particular location within the wing imaginal disc depends on the balance of Dpp to Gbb-60A signaling. Furthermore, the critical ratio of Gbb-60A to Dpp signaling appears to be mediated by both Tkv and Sax type I receptors.