An effective method for terrestrial arthropod euthanasia

Author:

Bennie Neil A.C.1,Loaring Christopher D.1,Bennie Mikaella M.G.1,Trim Steven A.1

Affiliation:

1. Venomtech Ltd, UK

Abstract

Summary As scientific understanding of invertebrate life increases so does the concern for how to end that life in a way that not only minimises (potential) suffering and is non-recoverable, but is also safe for those carrying out the procedure. There is increasing debate on the most appropriate euthanasia methods for invertebrates as their use in experimental research and zoological institutions grows. Their popularity as pet species has also lead to an increase in the need for greater veterinary understanding. Through the use of a local injection of potassium chloride (KCl) initially developed for use in American lobsters, this manuscript describes a safe and effective method for euthanasia in terrestrial invertebrates. Initial work focused on empirically determining the dose for cockroaches then extrapolating to other arthropod species. For this method of euthanasia, we propose the term 'targeted hyperkalosis' to describe death through terminal depolarisation of the thoracic ganglia as a result of high potassium concentration.

Publisher

The Company of Biologists

Subject

Insect Science,Molecular Biology,Animal Science and Zoology,Aquatic Science,Physiology,Ecology, Evolution, Behavior and Systematics

Reference9 articles.

1. American Veterinary Medical Association (2007). AVMA guidelines on euthanasia 2007. Available at https://www.avma.org/KB/Policies/Documents/euthanasia.pdf.

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3. Anaesthesia, analgesia and euthanasia of invertebrates;Cooper;ILAR J.,2011

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5. One scorpion, two venoms: prevenom of Parabuthus transvaalicus acts as an alternative type of venom with distinct mechanism of action;Inceoglu;Proc. Natl. Acad. Sci. USA,2003

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