Osmotic regulation in adultDrosophila melanogasterduring dehydration and rehydration

Author:

Albers Melissa A.1,Bradley Timothy J.1

Affiliation:

1. Department of Ecology and Evolutionary Biology, University of California, Irvine, CA 92612, USA

Abstract

SUMMARYWe have examined the osmoregulatory capacities of laboratory populations of the insect Drosophila melanogaster by measuring hemolymph osmotic concentration during desiccation and upon recovery from a bout of desiccation. Recovery treatments entailed allowing the flies access to distilled water, a saline solution or a saline+sucrose solution after a desiccation bout shown to reduce hemolymph volume by ∼60%. Prior to desiccation, the hemolymph osmotic concentration was 353±11 mOsm. We found that Drosophila display strict osmotic regulation under prolonged conditions of dehydration. Osmotic regulation continued during recovery from desiccation, regardless of the fluid provided. This result is evidence that this insect does not require an external source of osmolytes or energy to regulate its hemolymph osmotic concentration or to restore hemolymph volume,which is reduced during desiccation. We also examined populations that have been selected for over 250 generations for enhanced desiccation resistance to identify physiological characters that have evolved in response to the selection regime. The selected lines displayed a reduced pre-desiccation hemolymph osmotic concentration (315±7 mOsm) and a marginally improved capacity for osmoregulation.

Publisher

The Company of Biologists

Subject

Insect Science,Molecular Biology,Animal Science and Zoology,Aquatic Science,Physiology,Ecology, Evolution, Behavior and Systematics

Reference62 articles.

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2. Bosquet, G. (1977). Haemolymph modifications during starvation in Philosamia cynthia walkeri (Ferber) I. Volume,osmotic pressure, pH; relation to intracellular water content. Comp. Biochem. Physiol. A58,373-376.

3. Bradley, T. J. (1985). The excretory system:structure and physiology. In Comprehensive Insect Physiology,Biochemistry and Pharmacology, vol. 4 (ed. G. A. Kerkut and L. I. Gilbert), pp. 421-465. Elmsford, NY: Pergamon Press.

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