Auditory evoked potentials of utricular hair cells in the plainfin midshipman, Porichthys notatus

Abstract

The plainfin midshipman, Porichthys notatus, is a soniferous marine teleost fish that generates acoustic signals for intraspecific social communication. Nocturnally active males and females rely on their auditory sense to detect and locate vocally active conspecifics during social behaviors. Previous work showed that the midshipman inner ear saccule and lagena are highly adapted to detect and encode socially relevant acoustic stimuli, but the auditory sensitivity and function of the midshipman utricle remain largely unknown. Here, we characterize the auditory evoked potentials from hair cells in the utricle of non-reproductive type I males and test the hypothesis that the midshipman utricle is sensitive to behaviorally-relevant acoustic stimuli. Hair cell potentials were recorded from the rostral, medial and caudal regions of the utricle in response to pure tone stimuli presented by an underwater speaker. We show that the utricle is highly sensitive to particle motion stimuli produced by an underwater speaker positioned in the horizontal plane. Utricular potentials were recorded across a broad range of frequencies with lowest particle acceleration (dB re: 1 ms−2) thresholds occurring at 105 Hz (lowest frequency tested; mean threshold=-32 dB re: 1 ms−2) and highest thresholds at 605 to 1005 Hz (mean threshold range=−5 to −4 dB re: 1 ms−2). The high gain and broadband frequency sensitivity of the utricle suggests that it likely serves a primary auditory function and is well suited to detect conspecific vocalizations including broadband agonistic signals and the multiharmonic advertisement calls produce by reproductive type I males.