Interlocking of chelae is a key factor for dominance hierarchy formation in crayfish

Abstract

SUMMARY We characterized the role of chelae during agonistic encounters of the crayfish Procambarus clarkii. Physical asymmetries in body length, body mass and chelae size were directly related to dominance hierarchy formation. More than 80% of winning crayfish had longer body and chelae lengths, and winners were usually heavier in body mass, even if their differences were less than 3% compared with losing opponents. In mismatched pairings, large crayfish with short chelae were beaten by small crayfish with large chelae. Three physical elements of body length, body mass and chelae length equally affected the outcome of agonistic bouts. Chelae restriction, in which chelae were tightly closed using rubber bands, affected the outcome of agonistic bouts between large and small crayfish. Before chelae restriction, large crayfish won in all pairings. Following chelae restriction for at least 30 days from the first encounters, the winning rate of large crayfish that were previous winners decreased significantly in the second encounters against the same opponents that were previous losers. The handicap of chelae restriction significantly prolonged the time to formation of the winner–loser relationship. Individual fights escalated during agonistic bouts between large crayfish with one chela restricted and small crayfish with intact chelae, whereas the number of fights increased but the duration of individual fights did not increase in large crayfish with both chelae restricted. Furthermore, when the chelae of both large and small crayfish were disabled, the dominance order was frequently not formed during 30 min of agonistic bouts. Preventing chelae from interlocking prevented escalation of agonistic bouts. We show that interlocking of chelae acted as a key factor for the formation of dominance hierarchy.