Clearing of hemozoin crystals in malaria parasites enables whole-cell STED microscopy

Author:

Kehrer Jessica123ORCID,Pietsch Emma1ORCID,Heinze Julia1ORCID,Spielmann Tobias4ORCID,Frischknecht Friedrich12ORCID

Affiliation:

1. Integrative Parasitology, Center for Integrative Infectious Diseases, Heidelberg University Medical School 1 , Im Neuenheimer Feld 344, 69120 Heidelberg , Germany

2. German Center for Infection Research, DZIF, partner site Heidelberg 2 , 69120 Heidelberg , Germany

3. Infectious Diseases Imaging Platform, Center for Integrative Infectious Diseases, Heidelberg University Medical School 3 , Im Neuenheimer Feld 344, 69120 Heidelberg , Germany

4. Bernhard Nocht Institute for Tropical Medicine 4 , Bernhard Nocht Str. 74, 20359 Hamburg , Germany

Abstract

ABSTRACT Malaria is a devastating mosquito-borne parasitic disease that manifests when Plasmodium parasites replicate within red blood cells. During the development within the red blood cell, the parasite digests hemoglobin and crystalizes the otherwise toxic heme. The resulting hemozoin crystals limit imaging by STED nanoscopy owing to their high light-absorbing capacity, which leads to immediate cell destruction upon contact with the laser. Here, we establish CUBIC-P-based clearing of hemozoin crystals, enabling whole-cell STED nanoscopy of parasites within red blood cells. Hemozoin-cleared infected red blood cells could reliably be stained with antibodies, and hence proteins in the hemozoin-containing digestive vacuole membrane, as well as in secretory vesicles of gametocytes, could be imaged at high resolution. Thus, this process is a valuable tool to study and understand parasite biology and the potential molecular mechanisms mediating drug resistance. This article has an associated First Person interview with the first author of the paper.

Funder

Deutsches Zentrum für Infektionsforschung

Deutsche Forschungsgemeinschaft

Publisher

The Company of Biologists

Subject

Cell Biology

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Super-resolution microscopy: Shedding new light on blood cell imaging;Journal of Innovative Optical Health Sciences;2024-08-30

2. First person – Jessica Kehrer;Journal of Cell Science;2023-01-01

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