TORC1 and PKA activity towards ribosome biogenesis oscillates in synchrony with the budding yeast cell cycle

Author:

Guerra Paolo1ORCID,Vuillemenot Luc-Alban P. E.1ORCID,van Oppen Yulan B.1ORCID,Been Marije1ORCID,Milias-Argeitis Andreas1ORCID

Affiliation:

1. Molecular Systems Biology, Groningen Biomolecular Sciences & Biotechnology Institute, University of Groningen , 9747 AG Groningen , The Netherlands

Abstract

ABSTRACT Recent studies have revealed that the growth rate of budding yeast and mammalian cells varies during the cell cycle. By linking a multitude of signals to cell growth, the highly conserved target of rapamycin complex 1 (TORC1) and protein kinase A (PKA) pathways are prime candidates for mediating the dynamic coupling between growth and division. However, measurements of TORC1 and PKA activity during the cell cycle are still lacking. By following the localization dynamics of two TORC1 and PKA targets via time-lapse microscopy in hundreds of yeast (Saccharomyces cerevisiae) cells, we found that the activity of these pathways towards ribosome biogenesis fluctuates in synchrony with the cell cycle even under constant external conditions. Analysis of the effects of mutations of upstream TORC1 and PKA regulators suggests that internal metabolic signals partially mediate these activity changes. Our study reveals a new aspect of TORC1 and PKA signaling, which will be important for understanding growth regulation during the cell cycle.

Funder

Nederlandse Organisatie voor Wetenschappelijk Onderzoek

Rijksuniversiteit Groningen

Publisher

The Company of Biologists

Subject

Cell Biology

Reference100 articles.

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