Immune function is related to adult carotenoid and bile pigment levels, but not dietary carotenoid access during development, in female mallard ducks

Abstract

Summary Immune function can be modulated by multiple physiological factors, including nutrition and reproductive state. Because these factors can vary throughout an individual’s lifetime due to environmental conditions (e.g. nutrition) or life-history stage (e.g. adult reproduction), we must carefully examine the degree to which developmental versus adult conditions shape performance of the immune system. We investigated how variation in dietary access to carotenoid pigments – a class of molecules with immunostimulatory properties that females deposit into egg yolks – during three different developmental time points affected adult immunological and reproductive traits in female mallard ducks (Anas platyrhynchos). In males and females of other avian species, carotenoid access during development affects carotenoid assimilation ability, adult sexual ornamentation, and immune function, while carotenoid access at adulthood can increase immune response and reproductive investment (e.g. egg-laying capacity, biliverdin deposition in eggshells). We failed to find effects of developmental carotenoid supplementation on adult immune function (phytohemagglutinin-induced cutaneous immune response, antibody production in response to the novel antigen keyhole limpet hemocyanin [KLH], or oxidative burst, assessed by change in circulating nitric oxide levels), carotenoid-pigmented beak coloration, ovarian development, circulating carotenoid levels, or concentration of bile pigments in the gall bladder. However, we did uncover positive relationships between circulating carotenoid levels at adulthood and KLH-specific antibody production, and a negative relationship between biliverdin concentration in bile and KLH-specific antibody production. These results are consistent with the view that adult physiological parameters better predict current immune function than do developmental conditions and highlight a possible, previously unstudied relationship between biliverdin and immune system performance.