Affiliation:
1. University of Hohenheim, Institute of Physiology, 70593 Stuttgart, Germany and
2. Bayer AG, Agricultural Centre, MWF, Geb. 6240, Monheim, Germany
Abstract
SUMMARY
Amphibia have two classes of olfactory receptors (ORs), class I (fish-like receptors) and class II (mammalian-like receptors). These two receptor classes correspond to the two classes identified in other vertebrates, and amphibians thus provide a unique opportunity to compare olfactory receptors of both classes in one animal species, without the constraints of evolutionary distance between different vertebrate orders, such as fish and mammals. We therefore identified the complete open reading frames of class I and class II ORs in Xenopus laevis. In addition to allowing a representative comparison of the deduced amino acid sequences between both receptor classes, we were also able to perform differential functional analysis. These studies revealed distinct class-specific motifs, particularly in the extracellular loops 2 and 3, which might be of importance for the interaction with odorants, as well as in the intracellular loops 2 and 3, which might be responsible for interactions with specific G-proteins. The results of functional expression studies in Xenopus oocytes, comparing distinct receptor types, support the idea that class I receptors are activated by water-soluble odorants, whereas class II receptors are activated by volatile compounds.
Publisher
The Company of Biologists
Subject
Insect Science,Molecular Biology,Animal Science and Zoology,Aquatic Science,Physiology,Ecology, Evolution, Behavior and Systematics
Reference63 articles.
1. Altner, H. (1962). Untersuchungen über Leistungen und Bau der Nase des südafrikanischen Krallenfrosches Xenopus laevis (Daudin, 1803). Z. vergl. Physiol.45, 272–306.
2. Barth, A. L., Justice, N. J. and Ngai J. (1997). Asynchronous onset of odorant receptor expression in the developing zebrafish olfactory system. Neuron16, 23–34.
3. Ben-Arie, N., Lancet, D., Taylor, C., Khen, M., Walker, N., Ledbetter, D. H., Carrozzo, R., Patel, K., Sheer, D., Lehrach, H. and North, M. A. (1994). Olfactory receptor gene cluster on human chromosome 17: possible duplication of an ancestral receptor repertoire. Hum. Mol. Genet.3, 229–235.
4. Bozza, T. C. and Kauer, J. S. (1998). Odorant response properties of convergent olfactory receptor neurons. J. Neurosci.18, 4560–9.
5. Breer, H. and Benke, D. (1986). Messenger RNA from insect nervous tissue induces expression of neuronal acetylcholine receptors in Xenopus oocytes. Mol. Brain Res.1, 111–117.
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