Transcriptome sequencing reveals maelstrom as a novel target gene of the terminal-system in the red flour beetle Tribolium castaneum

Abstract

Terminal regions of the Drosophila embryo are patterned by the localized activation of the Torso-RTK pathway, which promotes the down-regulation of Capicua. In the short-germ beetle Tribolium, the function of the terminal system appears to be rather different, as the pathway promotes axis elongation and in addition, is required for patterning the extraembryonic serosa at the anterior. Here we show that Torso signalling induces gene expression by relieving CAPICUA-mediated repression also in Tribolium. Given that the majority of Torso target genes remain to be identified, we established a differential gene-expression screen. A subset of 50 putative terminal target genes was screened for functions in early embryonic patterning. Of those, 13 genes show early terminal expression domains and also phenotypes were related to terminal patterning. Among others, we found the PIWI-interacting RNA factor Maelstrom to be crucial for early embryonic polarization. Tc-mael is required for proper serosal size regulation and head morphogenesis. Moreover, Tc-mael promotes growth-zone formation and axis elongation. Our results suggest that posterior patterning by Torso may be realized through Maelstrom depended activation of posterior wnt-domains.