The C-terminus of the cargo receptor Erv14 affects COPII vesicle formation and cargo delivery

Author:

Lagunas-Gomez Daniel12,Yañez-Dominguez Carolina2,Zavala-Padilla Guadalupe2,Barlowe Charles3,Pantoja Omar2ORCID

Affiliation:

1. Centro de Investigación en Dinámica Celular, Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos 1 , Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos 62210 , Mexico

2. Instituto de Biotecnología, Universidad Nacional Autónoma de México 2 , Av. Universidad 2001, Cuernavaca, Morelos 62210 , México

3. Geisel School of Medicine, Dartmouth College 3 Department of Biochemistry , , Hanover, NH 03755-3844 , USA

Abstract

ABSTRACT The endoplasmic reticulum (ER) is the start site of the secretory pathway, where newly synthesized secreted and membrane proteins are packaged into COPII vesicles through direct interaction with the COPII coat or aided by specific cargo receptors. Little is known about how post-translational modification events regulate packaging of cargo into COPII vesicles. The Saccharomyces cerevisiae protein Erv14, also known as cornichon, belongs to a conserved family of cargo receptors required for the selection and ER export of transmembrane proteins. In this work, we show the importance of a phosphorylation consensus site (S134) at the C-terminus of Erv14. Mimicking phosphorylation of S134 (S134D) prevents the incorporation of Erv14 into COPII vesicles, delays cell growth, exacerbates growth of sec mutants, modifies ER structure and affects localization of several plasma membrane transporters. In contrast, the dephosphorylated mimic (S134A) had less deleterious effects, but still modifies ER structure and slows cell growth. Our results suggest that a possible cycle of phosphorylation and dephosphorylation is important for the correct functioning of Erv14.

Funder

Consejo Nacional de Ciencia y Tecnología

Universidad Nacional Autónoma de México

Publisher

The Company of Biologists

Subject

Cell Biology

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. First person – Daniel Lagunas-Gomez;Journal of Cell Science;2023-02-01

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