PQN-59 and GTBP-1 contribute to stress granule formation but are not essential for their assembly in C. elegans embryos

Author:

Abbatemarco Simona12,Bondaz Alexandra12,Schwager Francoise12,Wang Jing3,Hammell Christopher M.3,Gotta Monica12ORCID

Affiliation:

1. Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, 1211 Geneva, Switzerland

2. iGE3 Institute of Genetics and Genomics of Geneva, Geneva, Switzerland

3. Cold Spring Harbor Laboratory, New York, NY 11724, USA

Abstract

ABSTRACT When exposed to stressful conditions, eukaryotic cells respond by inducing the formation of cytoplasmic ribonucleoprotein complexes called stress granules. Here, we use C. elegans to study two proteins that are important for stress granule assembly in human cells – PQN-59, the human UBAP2L ortholog, and GTBP-1, the human G3BP1 and G3BP2 ortholog. Both proteins assemble into stress granules in the embryo and in the germline when C. elegans is exposed to stressful conditions. Neither of the two proteins is essential for the assembly of stress-induced granules, as shown by the single and combined depletions by RNAi, and neither pqn-59 nor gtbp-1 mutant embryos show higher sensitivity to stress than control embryos. We find that pqn-59 mutants display reduced progeny and a high percentage of embryonic lethality, phenotypes that are not dependent on stress exposure and that are not shared with gtbp-1 mutants. Our data indicate that, in contrast to human cells, PQN-59 and GTBP-1 are not required for stress granule formation but that PQN-59 is important for C. elegans development.

Funder

Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung

University of Geneva

Cold Spring Harbor Laboratory

Rita Allen Foundation

National Institute of General Medical Sciences

Publisher

The Company of Biologists

Subject

Cell Biology

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