Multi-Wavelength Spectrophotometric Analysis

Abstract

On the basis of the absorbance activity law, it is possible to carry out the spectrophotometric determination of two individual components (having totally or partially overlapping spectra) in a binary mixture. This determination can be carried out in a very precise and simple way by introducing ourselves in the multicomponent analysis by means of the so-called multi-wavelength linear regression analysis (MLRA) method that allows easy handling of data obtained at different wavelengths and whose results are comparable in accuracy and precision with other mathematical procedures. Another way of being able to calculate the individual concentration of two components is a mixture when their spectra are totally or partially overlapping; it is the derived spectrophotometry. The objective of this work is to apply the MLRA method for the individual determination of a binary mixture of metformin and sitagliptin as well as to apply derived spectrophotometry.