In the operation of manufacturing enterprise supply chain, there are lots of conflicts and differences between the node enterprises because of the different demands on the price, quality, cost, and other factors. These conflicts and differences can be effectively solved by negotiation. In this paper, the authors will abstract different entities in manufacturing enterprise supply chain as agents, and present a negotiation mode, and then discuss the negotiation tactics and procedures between the purchasing agent and supplier agent. Next, a practical example will be discussed and simulated for validating the negotiation model. Application of negotiation tactics and models will be helpful for resolving differences and conflicts, and improving negotiation efficiency. That will be used for optimizing the supply chain management, maximizing the benefits, and improving the operational efficiency of manufacturing enterprise supply chain.