Expression Characteristics and Sequence Variation Analysis of Rice Starch Regulator 1 Gene in Japonica Rice With Transgressive Variation

Abstract

The parents and transgressive variation lines of hybrids with significant difference in amylose content were selected to compare and analyze the accumulation characteristics of amylose and the change of OsRSR1 expression in grains in the process of grain filling, and the PCR technology was used to clone the OsRSR1 gene base sequence of four varieties. The results showed that the amylose content in grains increased gradually with grain filling process, the amylose content of offspring and parents with high amylose content were higher than the offspring and parents with low amylose content, hybrids could obtain the transgressive variation lines through the continuous directional selection of amylose content in grain, and the accumulation of amylose content in grain was closely related to genotypes. The expression quantity of OsRSR1 gene in grain was increasing during the grain filling process, the amylose content of grain was closely related to the activity of OsRSR1 gene, and the expression of grain OsRSR1 gene could also produce transgressive variation.