A NEW METHOD FOR OBTAINING HAPLOID PLANT SHED-MICROSPORTS CULTURE

Abstract

In plant breeding studies, dihaploidization method is used to obtain homozygous pure lines used to develop varieties in a shorter time. Dihaploidization method is applied in most types of vegetables such as wheat and corn, as well as in vegetable species. Androgenesis methods (anther and microspore culture) and gynogenesis and parthenogenesis methods (ovula and ovary culture) are used to obtain haploid plants. One of the androgenetic methods, shed-microspore culture has been a new alternative technique to anther and microspore cultures, and successful results have been obtained. The most important feature of this technique is the presence of a double-layered nutrient medium in a single petri dish, and the culture of microspores in the anthers in solid and liquid nutrient medium. In this study, the definition and application of microspore culture and shed-microspore cultures, studies conducted in the world and in our country and their developments in recent years were examined.