Celtis australis Exhibits Cytotoxic Effects by Inducing DNA Damage in Human Ovarian Cancer Cells

Author:

Fidan Gülsüm Nur1ORCID,Günay Sevilay2ORCID,Erden Yavuz3ORCID

Affiliation:

1. BARTIN UNIVERSITY

2. GAZI UNIVERSITY

3. Bartın University

Abstract

Native to the Mediterranean and southwest Asia, Celtis australis is used in folk medicine to treatment of many diseases such as peptic ulcer, diarrhea, dysentery, pain, and colic. However, there are very few studies on the effects of this species on cancer. We report for the first time the cytotoxic and genotoxic properties of C. australis on the human ovarian cancer cell line A2780. In the study, C. australis extract prepared in ethanol/phosphate buffer was applied to A2780 cells. The change in viability level in A2780 cells after treatment was determined by MTT assay and DNA damage was determined by single-cell gel electrophoresis (Comet) analysis. Our results showed that plant extract application at doses above 200 µg/ml significantly decreased A2780 cell viability (p

Funder

TÜBİTAK

Publisher

Bingol Universitesi

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