Investigation of Arginine deiminase activity in B. cereus and R. eutropha under minimal conditions

Abstract

Prokaryotes form an important part of our lives. These microorganisms, which we can not see with the naked eye, are used in medicine, biotechnology, microbiology and many other fields. They can produce many anti-cancer enzymes (Arginine deiminase, Asparaginase, Methionine gamma lyase etc.). In this study, an important bacterial anti-tumor enzyme of Bacillus cereus and R. eutropha in the presence of different minimal sources (Dextrose, Fructose, Glucose, Xylose, Maltose, Nutrient Broth Ramnose, Ribose, Sucrose) under static and shaking (150 rpm) conditions. Arginine deiminase activity was searched. In accordance with conclisions of our study, these microorganisms showed the highest ADI activity in the disaccharide medium, maltose and sucrose, and in the aldose group, in the ribose medium. It has been shown that this enzyme, which is used in cancer treatment, can be produced more cheaply and easily in minimal environments.