Serum biomarkers based neurotrauma severity scale: a study in the mice model of fluid percussion injury
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Published:2022-06-30
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ISSN:1689-0035
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Container-title:Acta Neurobiologiae Experimentalis
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language:
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Short-container-title:Acta Neurobiol Exp (Wars)
Author:
Aleem Mohd,Goswami Nidhi,Manda Kailash
Abstract
The study aimed to investigate the significance of serum biomarkers in the severity grading of traumatic brain injury (TBI). For this
purpose, mice underwent fluid percussion injury (FPI) at three discrete severity levels, mild, moderate, and severe. The severity
of trauma was verified by the qualitative and quantitative histopathology of the brain. The serum samples were analyzed for
the potential changes in ubiquitin C‑terminal hydrolase‑1 (UCHL‑1), S100β, interleukin‑6 (IL‑6), corticosterone, and β‑endorphin
at 24 and 72 h post injury. A multifold increase in the values of UCHL‑1 was reported at all severity extents of FPI. However,
TBI severity‑dependent increase in UCHL‑1 was reported on 72 h following FPI but not at 24 h. S100β values were significantly
augmented in the mild and moderate group at both the time point but not in the severe group. Serum level of IL‑6 was significantly
increased in the mild injury group at 24 h but not in the moderate and severe. At 72 h, IL‑6 showed a reverse trend. β‑endorphin
and corticosterone were sensitive at an early stage only. Such unique dynamics of each biomarker enable us to propose TBI
severity scale in the term of biomarkers codes to predict the extent of neurotrauma. Our preclinical study presents a predictive
model for further clinical validation.
Publisher
The Nencki Institute of Experimental Biology, Polish Academy of Sciences
Subject
General Medicine,General Neuroscience