In vitro callusogenesis and organogenesis of different Linum usitatissimum L. accessions

Abstract

Goal. The study of different genetic origin collection samples of flax (Linum usitatissimum L.) for the frequency and intensity of callusogenesis and organogenesis in vitro conditions, the establishment of differences in convar. elongatum, intermedia and humile was the goal of our research. Results and Discussion. The L. usitatissimum L. species is significantly capable of forming callus and shoot regeneration in vitro under cultivation conditions with a photoperiod of 16 hours, relative humidity of 60 – 80 %, air temperature of 22 – 24°С and agarized Murashige and Skoog medium, supplemented 0.05 mg/L of 1‑naphthylacetic acid, 1.0 mg/L of 6‑benzylaminopurine and 30 g/L of sucrose. The frequency and intensity of callusogenesis and organogenesis depends on the genotype. Minimum and maximum values of signs were set for Глінум (UF0401603), Кром (UF0401494), Орион (UF0401867), Есмань (UF0402071), Белита (UF0402134), Nor Man (UF0401792), Mapun M.A. (UF0401819), Lisa (UF0401830), Taragvi (UF0401864), Visamo (1–356)/L. monnseo (UF0402178), Ручеек (UF0401897), Lirina (UF0401900), Опус (UF0402142), СКі‑1 (UF0402143), Ruta (UF0402228) samples. The frequency of callusogenesis was 15.0 – 100 %, the calus mass from one explant was 0.56 – 1.51 g, the frequency of organogenesis was 10.0 – 93.8 %, the number of shoots was 1.4 – 4.0 pieces and the height of the shoots was 0.78 – 2.37 cm. Conclusions. Collection samples of Глінум (Ukraine), Кром (Russia), Visamo (1‑356)/L. monnseo (Czech Republic), Ручеек (Russia) and СКі‑1 (USA) were the best of complex signs (callusogenesis frequency, organogenesis frequency and number of shoots). Elongata flax and humile flax are characterized by the highest frequency of callusogenesis and organogenesis on hypocotyl and epicotyl explants, intermedia flax forms the largest mass of callus from the explant, the number of regenerated shoots and their height, intermedia flax has the greatest variation of the studied signs.