Nuclear-nucleolar relationships and nucleolar stress in hepatocytes in hyperhomocysteinemia

Abstract

Hyperhomocysteinemia causes stress of the endoplasmic reticulum, which suggests the formation of nucleolar stress. The purpose of this work is to clarify the relationship between structural changes in the nucleus and the region of the nucleolar organizer in hyperhomocysteinemia to prove nucleolar stress in hyperhomo-cysteinemia, which can serve as an additional diagnostic marker of the disease. The object of the study was white mongrel rats with methionine-induced hyperhomocysteinemia. Histologic sections of the liver were stained with hematoxylin and eosin (to assess the histological structure of the organ, hepatocyte nuclei), ammonia silver (to analyze the areas of the nucleolar organizer - AgNORs). Morphometry has allowed to establish that hyperhomocysteinemia decreases the number of nuclei (1,86 times, p