Norepinephrine regulates osteogenesis in the embryonic period of development

Abstract

Sympathetic and sensory nerve fibers regulate osteosynthesis and osteoresorption processes throughout life. Fundamental research and clinical data confirm the existence of functional interactions between neurons and bone tissue cells and indicate the catabolic and anabolic effect of sympathetic nervous system mediators on bone tissue. There is practically no information about the regulation of osteoremodeling in embryogenesis. Objective: to study the effect of norepinephrine on the growth of bone tissue explants in the embryonic period of development. The studies were performed on the bone tissue explants of 12-day old chicken embryos. Norepinephrine (10-10 M - 10-4 M), propranolol (10-10 M), atenolol (10-4 M), urapidil (10-6 M) were added to the experimental Petri dishes. Norepinephrine (10-6 M) stimulates the growth of the bone tissue explants through α1-adrenoreceptors. The osteotoxic effect of high doses of the drug is realized through β2-adrenoreceptors. Embryonic osteogenesis is regulated by norepinephrine in dose-dependent manner. Physiological effect of the substance depends on the interaction with certain types of adrenoreceptors.