Scanning Electron Microscopy in the Analysis of Species of the Genus Persicaria Mill

Author:

Gudkova A. A.1ORCID,Chistyakova A. S.1ORCID,Sinetskaya D. A.1ORCID,Slivkin A. I.1ORCID,Bolgov A. S.1ORCID,Bolgova M. A.2ORCID

Affiliation:

1. Voronezh State University

2. Municipal budgetary general education institution secondary general school No. 9

Abstract

Introduction.Scanning electron microscopy acts as a promising method of analysis, allowing to describe in detail the identification features of closely related plant species, as well as to conduct micro-roentgenstructural analysis of the sample in the selected area with high accuracy. The method is used to study the morphology of the surface and express determination of the composition of elements in various fields (physics, materials science, medicine, however, the use of the method of scanning/raster electron microscopy for the analysis of plant raw materials in pharmacy is limited.Aim.The purpose of the study was to study species of the genus Persicaria Mill. using scanning electron microscopy.Materials and methods.The objects of study were the Persicaria maculosa S.F. Gray, Persicaria hydropiper (L.) Delarbre, Persicaria tomentósa (Schrank) E.P. Bicknell, Persicaria mínor (Huds.) Opiz, Persicaria amphibia (L.) Delarbre, Persicaria amphibia var. terrestris (Leyss.) Munshi & Javeid, harvested during flowering in the Voronezh region. The study of samples by scanning electron microscopy was carried out on an electron microscope JSM-6510LV (Center for Collective Use of Voronezh State University).Results and discussion.With the help of scanning electron microscopy, morphological and anatomical signs of leaf plates of six species of the genus Persicaria were investigated and new diagnostic signs were identified. As a result of micro-X-RAY analysis, the content of some elements (potassium, calcium and magnesium) was established in the objects of study.Conclusion.For the first time to analyze the surface morphology of the leaf plate of species of the genus Persicaria Mill. the method of scanning electron microscopy is used. The features of the stomatal apparatus, trichomes and glands on the leaves of the studied species of Persicaria are clarified. With the help of micro-roentgenstructural analysis it was found, that a greater amount of magnesium is typical for the leaves of Persicaria mínor, calcium – for the leaves of Persicaria hydropiper, and potassium – for the leaves of Persicaria maculosa.

Publisher

Center of Pharmaceutical Analytics Ltd

Subject

Drug Discovery,Pharmaceutical Science

Reference26 articles.

1. Agapov B. L., Kulilova T. V. Rastrovaya electronnaya microscopiya [Scannig electron microscopy]. Voronezh: Izdatelskiy dom VGU; 2018. 28 p. (In Russ.)

2. Gudkova A. A., Chistyakova A. S., Sorokina A. A., Kuznetsov A. U., Khromykh E. G. Identification of representatives of the genus Persicaria Mill. by morphological characteristics. Pharmaciya. 2019;68(1):10–19. (In Russ.)

3. Kamalova U. B. Development of an algorithm for recognition of images of pollen grains obtained using a scanning electron microscope, and statistical analysis of their informative parameters. Vestnik IzhGTU. 2014;62(2):115–117. (In Russ.)

4. Dart P. J. Scanning Electron Microscopy of Plant Roots. Journal of Experimental Botany. 1976;70(22):163–168.

5. Kim S.-J., Kremer R. J. Scanning and transmission electron microscopy of root colonization of morningglory (Ipomoea spp.) seedlings by rhizobacteria. SYMBIOSIS. 2005;39(3):117–124.

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3