Coupling Michelson-like lateral shear interferometric microscopy with self-referencing numerical phase calibration for quantitative measurement of 3D surface morphology of biological cells

Abstract

A Michelson interferometer is commonly used for evaluating the morphology of a cell. However, the interference imaging with reference and object beams is easily affected by external vibrations and environmental disturbances, leading to unstable interference patterns. In this paper, the three-dimensional surface morphology of the biological cell is evaluated by a new quantitative phase imaging method, which couples Michelson-like lateral shear interferometric microscopy with self-referencing numerical phase calibration. The Michelson-like lateral shear interferometric microscopy is constructed by replacing the two plane mirrors of the traditional Michelson interferometer with two common right-angle prisms and generates interference fringe patterns. The lateral shear is created and freely adjustable by simply translating/or rotating one right-angle prism. To calculate the phase information of the biological cells quantitatively, the classical Fourier transform method is used to process the recorded interferogram, and then the self-referencing numerical phase calibration method is utilized for acquiring accurate phase information. Successfully achieving quantitative phase imaging of a cell verifies the feasibility and practicability of the proposed method.