THE DETERMINATION OF OLEIC ACID CONTENTS IN SUNFLOWER GENOTYPES

Abstract

High oleic sunflower is new trend both in Turkey and also in the world due to that it present healthy vegetable oil and also higher standing ability for frying. Higher oleic acid also affects from environment especially nigh temperatures during the grain filling period but genetic contribution is also important. High oleic acid content comes from Pervenent mutation in sunflower and it controlling Ol genes. However, because of being a seed trait which is determining after harvest, it is so difficult and unnecessary works (waiting even low oleic ones until seed treshing, etc) to select high oleic sunflower genetic materials. Therefore, selection utilization of molecular markers for determining of higher oleic types help breeders a lot to select accurately high oleic ones and also reduce costs both workers, isolation material, etc… The study covers determining of higher oleic type sunflower genetic materials developed in National Sunflower Hybrid Breeding Project conducted by Trakya Agricultural Research Institute. To screen of high oleic acid genotypes, around 400 sunflower F2 and F3 individuals obtained from crosses between high oleic acid and low oleic acid lines were used in TUBITAK (The Scientific and Technological Research Council of Turkey) Project 1003-114O971. Fatty acids of sunflower genotypes were determined by Agilent 6850 Gas Chromatography in Trakya University Lab. Based on the study results, oleic acid contents of sunflower genotypes were changed between 21.9-91.8 %, linoleic acid contents of them between 1.1-66.5 %, palmitic acid contents of them were between 3.4-8.0 % and stearic acid contents of genotypes were changed between 1.1-9.7 %. The higher oleic types were selected based on the study results for further generations.