Development and Validation of HPLC Method for Simultaneous Estimation of Minoxidil and Finasteride in Topical Solution-Reference-Cited by-同舟云学术

Development and Validation of HPLC Method for Simultaneous Estimation of Minoxidil and Finasteride in Topical Solution

Published:2023-12-30 Issue: Volume: Page:376-391
ISSN:2581-9429
Container-title:International Journal of Advanced Research in Science, Communication and Technology
language:en
Short-container-title:IJARSCT

Author:

Shradha S. Deshmukh ¹,Ajwitas Kale ¹

Affiliation:

1. P. Wadhwani College of Pharmacy, Yevatmal, India

Abstract

A simple, precise, rapid, accurate HPLC method has been developed and validated for the simultaneous determination of Minoxidil and Finasteride in pharmaceutical dosage form. The chromatographic separation was achieved on ODS C18 column (250mm*4.6mm,5 micrometer particle size) using a mobile phase comprising Buffer(7.0PH); ACN 80:20% v/v. The flow rate was 1ml/min and eluents were detected by UV detector at 210 nm. Retention times were found to be 2.967 min and 5.750 min Finasteride and Minoxidil respectively. The calibration curve was linear over the range of 20-80 microgram/ml of Minoxidil and 0.5 -1.6 microgram/ml of Finasteride. The developed method was successfully applied for determination of the two drugs from its pharmaceutical formulation. The excipients in the formulation do not pose any hindrance in determination of two drugs. The proposed method is suitable for routine quality control analysis

Publisher

Naksh Solutions

Subject

General Medicine

Reference80 articles.

1. A.A. Qureshi, N.A. Patel, A.R. Patel, "HPLC method development and validation for the simultaneous estimation of minoxidil and finasteride in topical solution," International Journal of Pharmacy and Pharmaceutical Sciences, 2014, 6(9): 449-452.

2. M. Ahmad, M. Abbas, S. Hussain, H. Akhtar, M.S. Arshad, M.A. Siddiqui, "Development and validation of RP-HPLC method for simultaneous estimation of minoxidil and finasteride in topical solution," Journal of Chromatographic Science, 2016, 54(7): 1131-1136.

3. V. Patil, V. Puranik, R. Rajput, "Development and validation of a reversed-phase high-performance liquid chromatographic method for simultaneous estimation of minoxidil and finasteride in topical solution," Journal of Applied Pharmaceutical Science, 2016, 6(4): 069-074.

4. S. Dhamija, M. Bhatia, A. Sharma, S. Sharma, "Development and validation of HPLC method for simultaneous estimation of minoxidil and finasteride in topical solution," International Journal of Research in Pharmacy and Pharmaceutical Sciences, 2017, 2(1): 26-32.

5. Al-khdhairawi, A. Zaidan, R. Tahir, "Development and validation of HPLC method for simultaneous estimation of minoxidil and finasteride in topical solution," European Journal of Biomedical and Pharmaceutical Sciences, 2018, 5(2): 162-168.