High-level heterologous protein production using an attenuated selection marker in Aspergillus sojae
Author:
Affiliation:
1. Research and Development Division, Kikkoman Corporation
Publisher
Microbiology Research Foundation
Subject
Applied Microbiology and Biotechnology,Microbiology
Link
https://www.jstage.jst.go.jp/article/jgam/67/2/67_2020.05.002/_pdf
Reference23 articles.
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2. Davies, R. W. (1991) Molecular biology of a high-level recombinant protein production system. In Molecular Industrial Mycology, 1st ed., ed. by Leong, S. A. and Berka, R. M., Marcel Dekker, New York, pp. 45–81.
3. Du, M., Battles, M. B., and Nett, J. H. (2012) A color-based stable multi-copy integrant selection system for Pichia pastoris using the attenuated ADE1 and ADE2 genes as auxotrophic markers. Bioeng. Bugs, 3, 32–37.
4. Erhart, E. and Hollenberg, C. P. (1983) The presence of a defective LEU2 gene on 2μ DNA recombinant plasmids of Saccharomyces cerevisiae is responsible for curing and high copy number. J. Bacterio., 156, 625–635.
5. Hynes, M. J. (1996) Genetic transformation of filamentous fungi. J. Genet., 75, 297-311.
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2. Heterologous production of ascofuranone and ilicicolin A in <i>Aspergillus sojae</i>;The Journal of General and Applied Microbiology;2022
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