Phenotypic difference between Δ(srl–recA)306 and ΔrecA::Km elucidated by next-generation sequencing combined with a long-PCR system
Author:
Affiliation:
1. Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine
2. Department of Biology, School of Biology, Tokai University
Publisher
Microbiology Research Foundation
Subject
Applied Microbiology and Biotechnology,Microbiology
Link
https://www.jstage.jst.go.jp/article/jgam/63/1/63_2016.07.002/_pdf
Reference38 articles.
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2. Baba, T. and Mori, H. (2008) The construction of systematic in-frame, single-gene knockout mutant collection in Escherichia coli K-12. Methods Mol. Biol., 416, 171–181.
3. Baba, T., Ara, T., Hasegawa, M., Takai, Y., Okumura, Y. et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol., 2, 2006 0008.
4. Bender, J. and Kleckner, N. (1992) Tn10 insertion specificity is strongly dependent upon sequences immediately adjacent to the target-site consensus sequence. Proc. Natl. Acad. Sci. USA, 89, 7996–8000.
5. Cao, Y. and Kogoma, T. (1995) The mechanism of recA polA lethality: suppression by RecA-independent recombination repair activated by the lexA(Def) mutation in Escherichia coli. Genetics, 139, 1483–1494.
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1. HslO ameliorates arrested ΔrecA polA cell growth and reduces DNA damage and oxidative stress responses;Scientific Reports;2022-12-23
2. Reactive oxygen species accumulation is synchronised with growth inhibition of temperature-sensitive recAts polA Escherichia coli;Archives of Microbiology;2022-06-16
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