Efficiency of bacteriological transport media quality control-Reference-Cited by-同舟云学术

Efficiency of bacteriological transport media quality control

Published:2021-06-07 Issue:6 Volume:66 Page:374-378
ISSN:2412-1320
Container-title:Russian Clinical Laboratory Diagnostics
language:
Short-container-title:Klin. lab. diagn.

Author:

Domotenko L. V.¹^ORCID,Morozova T. P.¹^ORCID,Shepelin A. P.¹^ORCID,Mironov A. Yu.²^ORCID

Affiliation:

1. State Research Center for Applied Microbiology and Biotechnology

2. G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology

Abstract

The aim of the study is to assess the effectiveness of quality control of transport environments in accordance with the requirements of domestic and international standards using aerobic and optional anaerobic microorganisms. The results of a comparative assessment of the survival of 10 strains of aerobic and optional anaerobic microorganisms after storage in the semi-liquid transport environment of Ames for 24 and 48 hours at temperatures (4-8)0 C and (20-25)0 C in accordance with the requirements of domestic and international standards are presented. The methodology described in ICC 4.2.2316 causes a number of technical difficulties leading to non-reproducible results. The survival rate of microorganisms in semi-liquid transport environments under ISO 11133-2016 is of high quality. The quantitative assessment is based on two Swab Elution and Roll Plate methods recommended by the CLSI M40-A2 standard.

Publisher

EKOlab

Subject

Biochemistry (medical),Medical Laboratory Technology,General Medicine

Reference16 articles.

1. World Health Organization SARS (Severe Acute Respiratory Syndrome) https://www.rospotrebnadzor.ru/upload/iblock/8e4/gosdoklad-za-2019_seb_29_05.pdf 2019. URL: https://www.who.int/ith/diseases/sars/en/ (Date of treatment 02/28/2021).

2. On the state of the sanitary-epidemiological well-being of the population in the Russian Federation in 2019: State report. Moscow: Rospotrebnadzor; 2020. URL: (Date of treatment 02/28/2021). (in Russian)

3. Men`shikov V.V. Why does the clinical laboratory need standardization and how to apply it in practice? Teaching aid. Moscow: Labora; 2012. (in Russian)

4. GOST R ISO 15189-2015. Medical laboratories. Requirements for quality and competence: Date of introduction 2016-06-01. URL: https://files.stroyinf.ru/Data2/1/4293764/4293764303.pdf (Date of treatment 02/28/2021). (in Russian)

5. Dyatlov I.A., Mironov A.Yu., Shepelin A.P., Alyoshkin V.A. The state and trends of clinical and sanitary microbiology in the Russian Federation and the problem of import substitution. Klinicheskaya laboratornaya diagnostika. 2015: 60 (8): 61-5. (in Russian)