Development of a PCR assay for the detection of human herpes virus type 7

Author:

Mardanly S. S.1ORCID,Mardanly S. G.1ORCID,Kazakov A. A.2ORCID,Demkin V. V.2ORCID,Zatevalov A. M.3ORCID,Mironov A. Yu.4ORCID

Affiliation:

1. CJSC «Ekolab»

2. Federal State Budgetary Institution "Institute of Molecular Genetics, National Research Center "Kurchatov Institute""

3. G.N. Gabrichevsky research institute for epidemiology and microbiology of the Rospotrebnadzor

4. G.N. Gabrichevsky research institute for epidemiology and microbiology of the Rospotrebnadzor; Federal Scientific and Clinical Center for Specialized Types of Medical Care and Medical Technologies of the FMBA of Russia

Abstract

A PCR assay has been developed to identify the DNA of the human herpes virus type 7. The search and selection of conserved regions was carried out by comparing the whole genome nucleotide sequences of HHV-7. A fragment duplicated in the HHV-7 genomes was chosen as a target for amplification. The performance of the assay was tested on a synthetic matrix and clinical samples. The developed assay has high sensitivity and specificity and showed good efficiency in detecting HHV-7 DNA in clinical samples.

Publisher

EKOlab

Subject

Biochemistry (medical),Medical Laboratory Technology,General Medicine

Reference13 articles.

1. Mardanly S.S., Mardanly S.G. Herpes virus type seven. literature review. Orekhovo-Zuevo: Gosudarstvennyi gumanitarno-tekhnologicheskiy universitet; 2021. (in Russian)

2. Salahuddin S.Z., Ablashi D.V., Markham P.D., Josephs S.F., Sturzenegger S., Kaplan M. et al. Isolation of a new virus, HBLV, in patients with lymphoproliferative disorders. Science. 1986; 234(4776): 596-601.

3. Medical Microbiology, Virology and Immunology: Textbook for Students of Medical Universities [Meditsinskaya mikrobiologiya, virusologiya I immunologiya]. Vorob`yov A.A., Bykov A.S., Boychenko M.N., eds. 3rd ed. Moscow: Meditsinskoe informatsionnoe agentstvo; 2022. ISBN 978-5-9986-0478-2 (in Russian)

4. Caserta M.T. 207 – Human Herpesviruses 6 and 7 (Roseola, Exanthem Subitum). Sarah S. Long, Charles G. Prober, Marc Fischer, eds. Principles and Practice of Pediatric Infectious Diseases. 4th ed. Elsevier. 2018; 1081-8e4. https://doi.org/10.1016/B978-0-323-40181-4.00207-3

5. Hall C.B., Long C.E., Schnabel K.C., Caserta M.T., McIntyre K.M., Costanzo M.A. et al. Human herpesvirus-6 infection in children. A prospective study of complications and reactivation. N. Eng. J.Med. 1994; 331: 432-8.

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