Development and validation of a methodology for quantitative determination of malondialdehyde by HPLC-MC/MS-Reference-Cited by-同舟云学术

Development and validation of a methodology for quantitative determination of malondialdehyde by HPLC-MC/MS

Published:2022-07-18 Issue:7 Volume:67 Page:369-373
ISSN:2412-1320
Container-title:Russian Clinical Laboratory Diagnostics
language:
Short-container-title:Klin. lab. diagn.

Author:

Mylnikov P. Yu.¹^ORCID,Shchulkin A. V.¹^ORCID,Abalenikhina Yuliya Vladimirovna¹^ORCID,Yakusheva E. N.¹^ORCID

Affiliation:

1. Ryazan State Medical University

Abstract

A bioanalytical technique for quantitative determination of MDA by HPLC-MS/MS. The proposed method for determining MDA includes the release stage of bound MDA and excludes the derivatization reaction. The lower limit of quantitative detection was 600 nmol/l, the volume of the required sample was 10 µl, the analysis time was 7 min. The range of concentrations obtained during the study makes it possible to use this bioanalytical technique to determine the concentration of MDA in biological material when assessing physiological and pathological conditions.

Publisher

EKOlab

Subject

Biochemistry (medical),Medical Laboratory Technology,General Medicine

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