Single-Tube Protocol for the Extraction of DNA or RNA from Paraffin-Embedded Tissues Using a Starch-Based Adhesive-Reference-Cited by-同舟云学术

Single-Tube Protocol for the Extraction of DNA or RNA from Paraffin-Embedded Tissues Using a Starch-Based Adhesive

Published:1996-05 Issue:5 Volume:20 Page:846-853
ISSN:0736-6205
Container-title:BioTechniques
language:en
Short-container-title:BioTechniques

Author:

Turbett Gavin R.¹,Barnett Timothy C.¹,Dillon Elizabeth K.¹²,Sellner Loryn N.¹

Affiliation:

1. Royal Perth Hospital, Perth, Australia

2. University of Western Australia, Nedlands, Australia

Abstract

We describe a method using an inexpenive craft glue to routinely isolate specific areas of tissue as small as 1 mm2 from paraffin sections. The tissue may be digested to release nucleic acid suitable for PCR or reverse transcription PCR. The use of this procedure obviates the requirement for manual microdissection or ultraviolet light irradiation. Tissue remaining on the slide can be stained and analyzed, allowing the precision of the extraction to be determined. The slide can be stored as a permanent record of the material taken for analysis.

Publisher

Future Science Ltd

Subject

General Biochemistry, Genetics and Molecular Biology,Biotechnology

Link

https://www.future-science.com/doi/pdf/10.2144/96205st04

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