Comparing robotic and manual injection methods in zebrafish embryos for high-throughput RNA silencing using CRISPR-RfxCas13d-Reference-Cited by-同舟云学术

Comparing robotic and manual injection methods in zebrafish embryos for high-throughput RNA silencing using CRISPR-RfxCas13d

Published:2024-02-29 Issue:5 Volume:76 Page:185-193
ISSN:0736-6205
Container-title:BioTechniques
language:en
Short-container-title:BioTechniques

Author:

Del Prado Joaquin Abugattas-Nuñez¹²^ORCID,Ding Yi³,Sonneville Jan de³^ORCID,der Kolk Kees-Jan van³,Moreno-Mateos Miguel A⁴⁵^ORCID,Málaga-Trillo Edward²^ORCID,Spaink Herman P¹^ORCID

Affiliation:

1. Institute of Biology Leiden, Animal Science & Health, Leiden University, Einsteinweg 55, Leiden, 2333CC, The Netherlands

2. Department of Biology, Universidad Peruana Cayetano Heredia, Av. Honorio Delgado 430, Lima, 15102, Perú

3. Life Science Methods BV, JH Oortweg 19, Leiden, 2333CH, The Netherlands

4. Andalusian Center for Developmental Biology (CABD), Pablo de Olavide University/CSIC/Junta de Andalucía, Ctra. Utrera Km.1, Seville, 41013, Spain

5. Department of Molecular Biology & Biochemical Engineering, Pablo de Olavide University, Ctra. Utrera Km.1, Seville, 41013, Spain

Publisher

Informa UK Limited

Link

https://www.tandfonline.com/doi/pdf/10.2144/btn-2023-0062

Reference38 articles.

1. Deep learning image recognition enables efficient genome editing in zebrafish by automated injections

2. An anti-tuberculosis compound screen using a zebrafish infection model identifies an aspartyl-tRNA synthetase inhibitor

3. A High-Throughput Screen for Tuberculosis Progression

4. Testing Tuberculosis Drug Efficacy in a Zebrafish High-Throughput Translational Medicine Screen

5. Robotic injection of zebrafish embryos for high-throughput screening in disease models

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