Establishment of cancer cell line originating from a patient with high-grade serous ovarian carcinoma-Reference-Cited by-同舟云学术

Establishment of cancer cell line originating from a patient with high-grade serous ovarian carcinoma

Published:2023-09 Issue:8 Volume:9 Page:
ISSN:2056-5623
Container-title:Future Science OA
language:en
Short-container-title:Future Science OA

Author:

Linh Nguyen Thuy¹²³^ORCID,Hang Ngo Thu¹,Cuong Bui Khac¹⁴^ORCID,Linh Dang Thuy¹,Phuong Linh Nham Thi⁴,Nguyen-Van Do⁵,Dzung Tran Ngoc²,Mao Can Van¹,Chung Dang Thanh²^ORCID,Chinh Le Tri⁶,Hung Nguyen Phu⁷,Tong Hoang Van¹⁸^ORCID,Toan Nguyen Linh¹^ORCID

Affiliation:

1. Department of Pathophysiology, Vietnam Military Medical University, Hanoi, Vietnam

2. Department of Pathology, Military Hospital 103, Vietnam Military Medical University, Hanoi, Vietnam

3. Department of Pathology, Hanoi Medical University Hanoi, Vietnam

4. Laboratory Animal Research Center, Vietnam Military Medical University, Hanoi, Vietnam

5. Department of Pathophysiology & Immunology, Hanoi Medical University, Hanoi, Vietnam

6. Department of Gynaecology Surgery, Vietnam National Cancer Hospital, Hanoi, Vietnam

7. Faculty of Biotechnology, Thai Nguyen University of Sciences, Thai Nguyen, Vietnam

8. Institute of Biomedicine & Pharmacy, Vietnam Military Medical University, Hanoi, Vietnam

Abstract

Aim: Ovarian cancer is a serious malignancy with high prevalence and mortality. Methods: We isolated and characterized an ovarian high-grade serous cancer cell line (M4) from a tumor of a Vietnamese patient with ovarian carcinoma. Results: The M4 cancer cell line showed good proliferation and stability in culture. Morphologically, the M4 cells showed similar characteristics to tumor cells such as a polyhedral shape, large irregular nuclei, high nuclear/cytoplasmic ratio, high nuclear density and expressing cancer markers like CA125, p53 and Ki67 markers. Conclusion: We have successfully isolated and characterized the M4 cell line from a Vietnamese patient with ovarian carcinoma.

Publisher

Future Science Ltd

Subject

Biotechnology

Link

https://www.future-science.com/doi/pdf/10.2144/fsoa-2023-0025

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