A modified mini-primer set for analyzing mitochondrial DNA control region sequences from highly degraded forensic samples-Reference-Cited by-同舟云学术

A modified mini-primer set for analyzing mitochondrial DNA control region sequences from highly degraded forensic samples

Published:2008-04 Issue:4 Volume:44 Page:555-558
ISSN:0736-6205
Container-title:BioTechniques
language:en
Short-container-title:BioTechniques

Author:

Young Lee Hwan¹,Young Kim Na¹,Jin Park Myung¹,Ick Yang Woo¹²,Shin Kyoung-Jin¹²

Affiliation:

1. Department of Forensic Medicine and Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine

2. Human Identification Research Center, Yonsei University, Seoul, Korea

Abstract

To facilitate the analysis of mitochondrial DNA (mtDNA) control region sequences from highly degraded skeletal remains, a modified mini-primer set was designed to overcome the limitations of the Armed Forces DNA Identification Laboratory (AFDIL) mini-primer set. This modified mini-primer set is less affected by nucleotide variability and PCR amplification conditions than the AFDIL mini-primer set, and was able to amplify the mtDNA sequences of 55-year-old skeletal remains with high efficiency, indicating that it is a useful tool for analyzing mtDNA control region sequences from highly degraded forensic samples.

Publisher

Future Science Ltd

Subject

General Biochemistry, Genetics and Molecular Biology,Biotechnology

Link

https://www.future-science.com/doi/pdf/10.2144/000112672

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