Single-step PCR-based genetic sex determination of rat tissues and cells-Reference-Cited by-同舟云学术

Single-step PCR-based genetic sex determination of rat tissues and cells

Published:2017-05 Issue:5 Volume:62 Page:232-233
ISSN:0736-6205
Container-title:BioTechniques
language:en
Short-container-title:BioTechniques

Author:

Dhakal Pramod¹,Soares Michael J.¹

Affiliation:

1. Institute for Reproductive Health and Regenerative Medicine, Department of Pathology & Laboratory Medicine and Department of Pediatrics, University of Kansas Medical Center, Kansas City, KS

Abstract

The advent of genome editing strategies has expanded the range of animal models available for gene manipulation and renewed research interest in the rat. Gender is a key variable for in vivo gene function analyses. Here, we present a simple PCR-based method to determine genetic sex in the rat.

Publisher

Future Science Ltd

Subject

General Biochemistry, Genetics and Molecular Biology,Biotechnology

Link

https://www.future-science.com/doi/pdf/10.2144/000114548

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