Epitope Tagging Genomic DNA Using a CD-Tagging Tn10 Minitransposon-Reference-Cited by-同舟云学术

Epitope Tagging Genomic DNA Using a CD-Tagging Tn10 Minitransposon

Published:2002-02 Issue:2 Volume:32 Page:422-430
ISSN:0736-6205
Container-title:BioTechniques
language:en
Short-container-title:BioTechniques

Author:

Telmer C.A.¹,Berget P.B.¹,Ballou B.¹,Murphy R.F.¹,Jarvik J.W.¹

Affiliation:

1. Carnegie Mellon University, Pittsburgh, PA, USA

Abstract

Here, we describe an efficient system for epitope tagging cloned genes by CD tagging using a mini-Tn10 transposon delivery vector. The system was tested against a λFIX genomic clone of the human nucleolin gene. Transfection of HeLa cells with the tagged gene led to the expression of both the appropriately spliced tagged transcript and the appropriately localized tagged protein.

Publisher

Future Science Ltd

Subject

General Biochemistry, Genetics and Molecular Biology,Biotechnology

Link

https://www.future-science.com/doi/pdf/10.2144/02322rr04

Cited by 13 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Fluorogen-activating proteins as biosensors of cell-surface proteins in living cells;Cytometry Part A;2010-06-07

2. A Modified Inverse PCR Procedure for Insertion, Deletion, or Replacement of a DNA Fragment in a Target Sequence and Its Application in the Ligand Interaction Scan Method for Generation of Ligand-Regulated Proteins;Methods in Molecular Biology;2010

3. Epitope tagging;BioTechniques;2008-04

4. Automated interpretation of subcellular patterns in fluorescence microscope images for location proteomics;Cytometry Part A;2006

5. Automated Interpretation of Protein Subcellular Location Patterns;International Review of Cytology;2006