Affiliation:
1. Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna, via Bianchi 9, 25124, Brescia, Italy
Abstract
Routine cell culture demands the use of animal-derived products, mainly fetal bovine serum and swine or bovine trypsin. According to the 3Rs principle and to the European Centre for the Validation of Alternative Methods, animal-free substitutes are strongly recommended for in vitro methods. In this study, the HEp-2 cell line was adapted to different totally animal-free culture systems, such as a serum-free complete medium (VP-SFM), human platelet lysate and a synthetic trypsin (TrypLE™ Express); afterward, cell growth was assessed with the xCELLigence instrument. Animal-free products provided promising results, with performances similar or preferable to the common reagents; therefore their use could be encouraged for both ethical and technical advantages.
Funder
Italian Ministry of Health
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna
Subject
General Biochemistry, Genetics and Molecular Biology,Biotechnology
Cited by
4 articles.
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