tANCHOR: a novel mammalian cell surface peptide display system-Reference-Cited by-同舟云学术

tANCHOR: a novel mammalian cell surface peptide display system

Published:2021-01 Issue:1 Volume:70 Page:21-28
ISSN:0736-6205
Container-title:BioTechniques
language:en
Short-container-title:BioTechniques

Author:

Ivanusic Daniel¹,Madela Kazimierz²,Burghard Heidi¹,Holland Gudrun²,Laue Michael²,Bannert Norbert¹

Affiliation:

1. FG18 HIV & Other Retroviruses, Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany

2. ZBS 4 Centre for Biological Threats & Special Pathogens: Advanced Light & Electron Microscopy, Robert Koch Institute, Nordufer 20, 13353 Berlin, Germany

Abstract

A novel tool for the presentation of peptides and small proteins on the surface of human cells has been developed. Our tANCHOR system utilizes tetraspanin anchors containing heterologous amino acid sequences inserted instead of the large extracellular loop. This technology allows a highly effective extracellular display of epitopes for antibody binding studies and many other potential applications.

Publisher

Future Science Ltd

Subject

General Biochemistry, Genetics and Molecular Biology,Biotechnology

Link

https://www.future-science.com/doi/pdf/10.2144/btn-2020-0073

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1. CD81 fusion alters SARS-CoV-2 Spike trafficking;mBio;2024-09-11

2. tANCHOR-cell-based assay for monitoring of SARS-CoV-2 neutralizing antibodies rapidly adaptive to various receptor-binding domains;iScience;2024-03

3. tANCHOR cell-based ELISA approach as a surrogate for antigen-coated plates to monitor specific IgG directed to the SARS-CoV-2 receptor-binding domain;Biology Methods and Protocols;2024-01-01

4. tANCHOR fast and cost-effective cell-based immunization approach with focus on the receptor-binding domain of SARS-CoV-2;Biology Methods and Protocols;2023-01-01

5. Establishment of a human cell line with a surface display system for screening and optimizing Na+-taurocholate cotransporting polypeptide-binding peptides;Frontiers in Microbiology;2022-08-17