Deoxyribonuclease Treatment Improves the Homogeneity of Single-Stranded DNA Preparations
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Published:1997-01
Issue:1
Volume:22
Page:105-110
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ISSN:0736-6205
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Container-title:BioTechniques
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language:en
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Short-container-title:BioTechniques
Author:
Aliev T.K.1,
Panina A.A.1,
Korobko V.G.1,
Varfolomeyev S.D.1
Affiliation:
1. Moscow State University
Abstract
The isolation of single-stranded (ss) phagemid DNA using standard protocols often results in impure preparations, which contain undesirable quantities of chromosomal and/or double-stranded (ds) phagemid DNA. Here we report a simple and efficient method for elimination of virtually all dsDNA by incubation of phagemid viral particles with deoxyribonuclease I. In addition to analyzing the ratio of linear-tocircular topological forms of ssDNA after deoxyribonuclease I treatment, we verified that no decrease in transformation efficiency occurred and demonstrated that ssDNA molecules covered by capsid proteins remained intact following such treatment.
Publisher
Future Science Ltd
Subject
General Biochemistry, Genetics and Molecular Biology,Biotechnology