Fabrication of Microarray of Gel-Immobilized Compounds on a Chip by Copolymerization

Author:

Vasiliskov A.V.12,Timofeev E.N.13,Surzhikov S.A.13,Drobyshev A.L.132,Shick V.V.13,Mirzabekov A.D.132

Affiliation:

1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia

2. Moscow Institute of Physics and Technology, Dolgoprudny, Russia

3. Argonne National Laboratory, Argonne, IL, USA

Abstract

The manufacturing of microchips containing oligonucleotides and proteins immobilized within gel pads, ranging in size from 10 × 10 to 100 × 100 μm, is described. The microchips are produced by photo- or persulfate-induced copolymerization of unsaturated derivatives of biomolecules with acrylamide-bisacrylamide mixture. Oligonucleotides containing 5′-allyl or 5′-butenediol units were synthesized using standard phosphoramidite chemistry. Acryloyl residues were attached to a protein by a twostep procedure. Photopolymerization was induced by illumination of the monomer solution containing initiator with UV light through the mask. The mask was applied directly over the monomer solution or projected through a microscope. Alternatively, copolymerization was carried out in drops of aqueous solution of monomers containing ammonium persulfate. Drops with different allyl-oligonucleotides were distributed on a glass slide, and the polymerization was induced by diffusion of N,N,N′,N′-tetramethylethylenediamine (TEMED) from a hexane solution that covered the aqueous drops.

Publisher

Future Science Ltd

Subject

General Biochemistry, Genetics and Molecular Biology,Biotechnology

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3